Airplane - which features classic one liners such as 'I am serious and don't call me Shirley' - came out top in research conducted by a panel of members of movie subscription service Lovefilm.
They calculated the precise number of 'laughs a minute' for the top 10 comedies chosen by Lovefilm members.
The panel recorded the total number of times each film generated a laugh, before dividing it by the films' total length in minutes to calculate the precise 'laugh a minute' rating for each movie.
With a 'laugh A minute' score of three, Airplane beat nine rival comedies to top the list which has been created to mark The Hangover becoming available to stream on Lovefilm Instant.
The Naked Gun: From the Files of Police Squad completed the top three with a 'laugh a minute' score of 2.3.
Foul-mouthed Superbad managed a 'laugh a minute' score of 1.9, followed closely by the outrageous and least politically correct movie ever made, Borat, with 1.7.
Modern classic Anchorman reported in at sixth place. This battle of the sexes in a newsroom has spawned a whole range of one liners and manages to make audiences laugh 1.6 times per minute on average.
Helen Cowley, editor of Lovefilm, said: "After many heated debates about whether The Hangover really is the funniest movie ever, we asked our members to vote for the 10 movies that make them laugh the most, and then conducted our own research into how many times those included in this list made us giggle.
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